A Taste of Genetics MiniLab

Genetics gets personal as students extract and amplify their own DNA, then utilize PCR and electrophoresis to determine their genotype for the PTC tasting trait using a restriction digest assay.

Description
A comprehensive kit for 10 student groups to extract & amplify their own DNA with PCR and then do a restriction digest assay to determine their genotype.

Teaching AP Biology? A Taste of Genetics MiniLab and extension activities are three Big Ideas in one comprehensive package. Together, these labs are an in-depth exploration of the TAS2R38 gene, covering hands-on genetic analysis, bioinformatics, population genetics, and evolution.Materials Included in each MiniLab:
Contains enough of the following for 10 student groups, 2 – 3 students per group.

• Ten 2% Agarose GreenGel™ Cups
• One bottle of 100 mL TBE buffer concentrate
• DNA extraction solution
• Forward and reverse primers for PTC genes
• Taq polymerase master mix (2X)
• HaeIII restriction enzyme
• Restriction enzyme dilution buffer
• MiniOne® Sample Loading Dye (5X)
• MiniOne® Molecular Weight Marker
• One bag of 0.2mL thin-walled PCR tubes
• One bag of 0.65 mL microcentrifuge tubes
• Forty PTC taste strips
• Ten racks of micropipette tips, 96 tips/rack, 1-200µL