Nalgene Rapid-Flow Sterile Disposable Filter Units
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Thermo Scientific™ Nalgene™ Rapid-Flow™ Sterile Disposable Filter Units
Bottle Top Filter Units and Storage Bottles Only Nalgene has the unique Rapid-Flow™ membrane support system that uses an evenly-spaced array of columns to provide greater membrane stability – resulting in faster flow and higher throughput of fluids – perfectly suited for sterile filtration and clarification of 50-1000 mL of cell culture media, serum, additives and buffers.
Rapid-Flow Selection Guide
| Making the Right Selection | |
| Select the right pore size |
– 0.1 micron for sterile filtration and Mycoplasma removal – 0.2 micron for sterile filtration – 0.45 micron for clarification – 0.8 micron for prefiltration and clarification |
| Select the right capacity | Choose from 50 mL, 115 mL, 150 mL, 250 mL, 500 mL, 1000 mL capacity filter units and bottle top filters. |
| Select the right membrane diameter | Options include 50 mm, 75 mm and 90 mm diameter. Wider membranes mean faster flow and more throughput so you don’t have to reach for a second filter for difficult-to-filter fluids. Only Nalgene has 90 mm diameter membranes in 500 mL and 1000 mL size filter units and bottle top filters. |
| Select the right membrane |
– PES (polyethersulfone) is the ultimate cell culutre membrane with low protein binding so there is less chance of removing critical protein from your media. It is hydrophylic, so no external wetting agents or surfactants are needed, resulting in low extractables. And, it’s fast so you spend less time waiting. – SFCA (surgactant-free cellulose acetate) containes none of the wetting agents founf in standard CA (cellulose acetate), and also has lower protein binding than standard CA. If you use CA for medie filtration you owe it to your cell lines to switch to SFCA; it’s only available in Nalgene filter units and bottle top filters. – CN (cellulose acetate) is ideal for filtering water, buffers and other aqueous solutions when protein binding is not a concern or if you want to remove extraneous protein. – NYL (nylon) membranes are tough, alcohol resistant and have low levels of extractables. Choose them for filtering alcoholic solutions. |
| Select the right media storage bottle | Nalgene storage bottle cap design prevents gas exchange and maintains pH of stored media for up to two weeks. Nalgene storage bottles are available in 150, 250, 500 and 1000 mL sizes, separately or as an integral part of all Nalgene filter units. |
| Select the right quality | Every lot is Nalgene-Certified to be sterile, non-cytotoxic, non-pyrogenic, and to have passed strict performance tests. We guarantee it! |
Thermo Scientific™ Nalgene™ Rapid-Flow Sterile Disposable Filter Units – PES Membrane
Nalgene™ Rapid-Flow disposable Filter Units with PES (polyethersulfone) membrane provide the last line of defense against cell culture contamination.
Low protein binding PES membranes with low extractables are the best choice for sterile filtration of cell culture media, serum, additives and buffers. Asymmetric PES membranes and exclusive Rapid-Flow support plate design provide fast flow rates and high throughput for increased filtration efficiency.
details
- Filter cup unscrews from storage bottle for easy access to filtrate
- Leakproof† (except 115 mL sizes) screw cap closure eliminates pH shift in storage bottle for longer life of stored media
- Upper chamber of filter units is clearly marked with pore size, membrane type, catalog number, lot number and expiration date (except 115 mL size)
- Padless membrane support minimizes foaming of proteinaceous samples
- Non-pyrogenic and non-cytotoxic
- PES membrane with blue collar
- Gamma radiation-sterilized and individually bagged for a 5-year sterile shelf life
Polyethersulfone (PES) Membrane
- PES is the best membrane for cell culture fluids; lowest protein binding to maintain protein balance, lowest extractables to maintain media purity
- Asymmetric PES membrane plus unique Rapid-Flow support plate design provides fast flow and high throughput of fluids for increased filtration efficiency
- 0.2 μm units protect against bacterial contamination
- 0.1 μm units also protect against Mycoplasma contamination
115 mL Sizes
- Compact filter units for quick filtration of 50-100 mL of fluid
- Unitary construction with non-separable upper and lower compartments
- Not intended for post-filtration storage of media (use Rapid-Flow products if media storage is required)
Includes: Polystyrene cover; graduated upper chamber and storage bottle (except 115 mL size); integral membrane filter; cellulosic-plugged side arm; quick-disconnect tubing adapter.
† The term “leakproof” applies to Thermo Scientific Nalgene products that meet the following criteria: a) Bottle/flask/funnel closure systems with closures smaller than 100 mm, after they are filled with water, inverted, withstand air pressure of 2psig for 2 minutes, and no water escapes;. b) Bottle/funnel/flask closure systems of larger than 100 mm after they are filled with water, inverted for 15 minutes, and no water escapes. Note: these tests, using other liquids, may not yield the same results. To ensure safe usage, customers are advised to test Thermo Scientific Nalgene bottles and closures under conditions of their planned applications. Thermo Scientific Nalgene products are leakproof at ambient temperature and pressure when used with their Nalgene closures.
Nalgene™ Rapid-Flow Sterile Disposable Filter Units
| Cat. No. | Capacity, Upper/Storage Bottle (mL) |
Material (Membrane) |
Pore Size (μm) |
Membrane Dia (mm) |
No. per Case |
| 565-0010 | 150/150 | PES | 0.1 | 50 | 12 |
| 568-0010 | 250/250 | PES | 0.1 | 50 | 12 |
| 566-0010 | 500/500 | PES | 0.1 | 75 | 12 |
| 567-0010 | 1000/1000 | PES | 0.1 | 90 | 12 |
| 564-0020 | 150/50 | PES | 0.2 | 50 | 12 |
| 524-0020* | 115/115 | PES | 0.2 | 50 | 72 |
| 565-0020 | 150/150 | PES | 0.2 | 50 | 12 |
| 568-0020 | 250/250 | PES | 0.2 | 50 | 12 |
| 566-0020 | 500/500 | PES | 0.2 | 75 | 12 |
| 569-0020 | 500/500 | PES | 0.2 | 90 | 12 |
| 567-0020 | 1000/1000 | PES | 0.2 | 90 | 12 |
| 124-0045* | 115/115 | PES | 0.45 | 50 | 72 |
| 165-0045 | 150/150 | PES | 0.45 | 50 | 12 |
| 168-0045 | 250/250 | PES | 0.45 | 50 | 12 |
| 166-0045 | 500/500 | PES | 0.45 | 75 | 12 |
| 169-0045 | 500/500 | PES | 0.45 | 90 | 12 |
| 167-0045 | 1000/1000 | PES | 0.45 | 90 | 12 |
* 115 mL sizes do not have Rapid-Flow support plate design
Thermo Scientific™ Nalgene™ Rapid-Flow Sterile Disposable Bottle Top Filters – PES Membrane
Nalgene™ MF75 disposable Bottle Top Filters with PES (polyethersulfone) membrane provide the last line of defense against cell culture contamination.
Low protein binding PES membranes with low extractables are the best choice for sterile filtration of cell culture media, serum, additives and buffers. Asymmetric PES membranes and exclusive Rapid-Flow support plate design provide fast flow rates and high throughput for increased filtration efficiency.
details
- Filters marked with catalog number, membrane type and pore size, lot number and expiration date for easy traceability
- Gamma radiation sterilized and individually bagged for a 5-year sterile shelf life
- Molded-in graduations for easy visualization of sample size
- Side-arm with quick-disconnect tubing adapter for easy change between filters
- Use with Nalgene storage bottles with catalog numbers starting with 455- or glass bottles rated for vacuum
- Non-cytotoxic and non-pyrogenic
Polyethersulfone (PES) membranes
- PES is the best membrane for cell culture fluids; lowest protein binding to maintain protein balance, lowest extractables to maintain media purity
- Asymmetric PES membrane plus unique Rapid-Flow support plate design provide fast flow and high throughput of fluids for increased filtration efficiency
- Use 0.2 μm membranes for sterile filtration, 0.45 μm membranes for fluid clarification and particle removal
Includes: Polystyrene graduated chamber; Blue color-coded collar with cellulosic vent plug side arm and quick-disconnect tubing adapter; 50, 75, or 90 mm
diameter membranes; bottom screw threads for attachment to bottles with 33 or 45 mm neck sizes.
CAUTION: Use only sterile bottles designed for use with vacuum. Always use safety shield during vacuum procedures.
Nalgene Rapid-Flow Sterile Disposable Bottle-Top Filters
| Cat. No. | Capacity (ml) |
Fits Bottle Neck Size (mm) |
Material (Membrane) |
Pore Size (μm) |
Membrane Dia. (mm) |
No. per Case |
| 596-3320 | 150 | 33 | PES | 0.2 | 50 | 12 |
| 596-4520 | 150 | 45 | PES | 0.2 | 50 | 12 |
| 296-3345 | 150 | 33 | PES | 0.45 | 50 | 12 |
| 296-4545 | 150 | 45 | PES | 0.45 | 50 | 12 |
| 595-3320 | 500 | 33 | PES | 0.2 | 75 | 12 |
| 595-4520 | 500 | 45 | PES | 0.2 | 75 | 12 |
| 295-3345 | 500 | 33 | PES | 0.45 | 75 | 12 |
| 295-4545 | 500 | 45 | PES | 0.45 | 75 | 12 |
| 597-3320 | 1000 | 33 | PES | 0.2 | 90 | 12 |
| 597-4520 | 1000 | 45 | PES | 0.2 | 90 | 12 |
Thermo Scientific™ Nalgene™ Rapid-Flow Sterile Disposable Filter Units SFCA Membrane
Nalgene™ Rapid-Flow™ Sterile Disposable Filter Units with SFCA Membrane are ideal for vacuum filtration and single-use biological work requiring sterile filtration, prefiltration or clarification of aqueous solutions in the lab or field.
Surfactant-Free Cellulose Acetate (SFCA) membranes have lower extractables than standard (CA) membranes. If you use cellulose acetate (CA) for filtration of media or other cell culture fluids, you owe it to your cells to switch to cleaner, safer Nalgene SFCA.
details
- Nalgene Rapid-Flow membrane support system provides fast flow and high throughput
- Upper chamber separates from storage bottle for easy access to filtrate
- Leakproof† (except 115 mL sizes) crew cap eliminates pH shift in storage bottle to provide longer life for stored media
- Upper chamber of filter units is clearly marked with pore size, membrane type, catalog number, lot number and expiration date (except 115 mL size)
- Padless membrane support minimizes foaming of proteinaceous samples
- Non-pyrogenic and non-cytotoxic
- Yellow collar for easy identification of SFCA membrane
- Gamma radiation-sterilized and individually bagged for a 5-year sterile shelf life
Surfactant-free cellulose acetate membranes (SFCA)
- Low protein binding, excellent flow rates, little or no loss of specific proteins
- No wetting agents to affect sensitive cell culture lines
- Much lower extractables than standard CA membranes makes SFCA a better choice for cell culture
115 mL sizes
- Compact filter units for quick filtration of 50-100 mL of fluid
- Unitary construction with non-separable upper and lower compartments
- Not intended for post-filtration storage of media (use Rapid-Flow products if media storage is required)
Includes: Polystyrene cover; graduated upper chamber and storage bottle (except 115 mL size; integral membrane filter; cellulosic-plugged side arm; quick disconnect tubing adapter.
Nalgene™ Rapid-Flow Sterile Disposable Filter Units
| Cat. No. | Capacity, Upper/Storage Bottle (mL) |
Material (Membrane) |
Pore Size (μm) |
Membrane Dia (mm) |
No. per Case |
| 122-0020* | 115/115 | SFCA | 0.2 | 50 | 72 |
| 155-0020 | 150/150 | SFCA | 0.2 | 50 | 12 |
| 157-0020 | 250/250 | SFCA | 0.2 | 50 | 12 |
| 156-4020 | 500/500 | SFCA | 0.2 | 75 | 12 |
| 158-0020 | 500/1000 | SFCA | 0.2 | 75 | 12 |
| 162-0020 | 500/500 | SFCA | 0.2 | 90 | 12 |
| 161-0020 | 1000/1000 | SFCA | 0.2 | 90 | 12 |
| 122-0045* | 115/115 | SFCA | 0.45 | 50 | 72 |
| 155-0045 | 150/150 | SFCA | 0.45 | 50 | 12 |
| 157-0045 | 250/250 | SFCA | 0.45 | 50 | 12 |
| 158-0045 | 500/1000 | SFCA | 0.45 | 75 | 12 |
| 156-4045 | 500/500 | SFCA | 0.45 | 75 | 12 |
| 162-0045 | 500/500 | SFCA | 0.45 | 90 | 12 |
| 161-0045 | 1000/1000 | SFCA | 0.45 | 90 | 12 |
* 115 mL sizes do not have Rapid-Flow support plate design
Thermo Scientific™ Nalgene™ Rapid-Flow Sterile Disposable Bottle Top Filters
SFCA Membrane
Nalgene™ Rapid-Flow™ Sterile Disposable Bottle-Top Filters fit Nalgene filter receiver bottles (#455-series) or standard glass media bottles.
Surfactant-Free Cellulose Acetate (SFCA) membranes have lower extractables than standard cellulose acetate (CN) membranes. If you use CA for filtration of media or other cell culture fluids, you owe it to your cells to switch to cleaner, safer Nalgene SFCA.
details
- Nalgene Rapid-Flow membrane support system provides fast flow and high throughput
- Filters marked with catalog number, membrane type and pore size, lot number and expiration date for easy traceability
- Gamma radiation sterilized and individually bagged for 5-year sterile shelf life
- Molded-in graduations for quick visualization of sample volume
- Side-arm with quick-disconnect tubing adapter for easy bottle top changes
- Non-cytotoxic and non-pyrogenic
Surfactant-free cellulose acetate membranes (SFCA)
- Low protein binding, excellent flow rates, little or no loss of specific proteins
- No wetting agents to affect sensitive cell culture lines
- Much lower extractables than standard CA membranes makes SFCA a better choice for cell culture
Includes: Polystyrene graduated chamber; color-coded collar with cellulosic vent plug side arm and quick-disconnect tubing adapter; 50, 75, or 90 mm diameter membranes; bottom screw threads for attachment to bottles with 33 or 45 mm neck sizes.
Nalgene Rapid-Flow Sterile Disposable Bottle Top Filters
| Cat. No. | Capacity (ml) |
Fits Bottle Neck Size (mm) |
Material (Membrane) |
Pore Size (μm) |
Membrane Dia. (mm) |
No. per Case |
| 290-3320 | 150 | 33 | SFCA | 0.2 | 50 | 12 |
| 290-3345 | 150 | 33 | SFCA | 0.45 | 50 | 12 |
| 290-4520 | 150 | 45 | SFCA | 0.2 | 50 | 12 |
| 290-4545 | 150 | 45 | SFCA | 0.45 | 50 | 12 |
| 291-3320 | 500 | 33 | SFCA | 0.2 | 75 | 12 |
| 291-3345 | 500 | 33 | SFCA | 0.45 | 75 | 12 |
| 291-4520 | 500 | 45 | SFCA | 0.2 | 75 | 12 |
| 291-4545 | 500 | 45 | SFCA | 0.45 | 75 | 12 |
| 292-3320 | 1000 | 33 | SFCA | 0.2 | 90 | 12 |
| 292-4520 | 1000 | 45 | SFCA | 0.2 | 90 | 12 |
Thermo Scientific™ Nalgene™ Rapid-Flow Sterile Disposable Filter Units CN Membrane
Nalgene™ Rapid-Flow Sterile Disposable Filter Units with CN (Cellulose Nitrate) membrane are ideal for sterile filtration, prefiltration or clarification of buffers and other aqueous solutions when protein
binding is not a concern. Nalgene CN membranes are Triton™-free
details
- Nalgene Rapid-Flow membrane support system provides fast flow and high throughput
- Upper chamber separates from storage bottle for easy access to filtrate
- Leakproof† screw cap eliminates pH shift in storage bottle to provide longer life for stored media
- Upper chamber of filter units is clearly marked with pore size, membrane type, catalog number, lot number and expiration date for traceablility
- Padless membrane support minimizes foaming of proteinaceous samples
- Non-pyrogenic and non-cytogenic
- Green collar for easy identification of CN membrane
- Gamma radiation-sterilized and individually wrapped for a 5-year sterile shelf life
115 mL Sizes
- Compact filter units for quick filtration of 50 to 100 mL of fluid
- Unitary construction with non-separable upper and receiver components
- Not intended for post-filtration storage of media (use Rapid-Flow products if media storage is required)
Includes: 50 or 75 mm diameter membrane, polyethylene adapter, and quickdisconnect tubing adapter. Each pack of 500 and 1000 mL units includes 12 glass fiber prefilters. All sizes except 115 mL supplied with sterile polyethylene leakproof† cap.
Notes: CN membranes are best used for filtration of buffers and general lab solutions. We do not recommend CN for filtration of cell culture fluids because it has high protein binding characteristics. Use PES or SFCA for cell culture media, serum, additives, etc.
Nalgene™ Rapid-Flow Sterile Disposable Filter Units
| Cat. No. | Capacity, Upper/Storage Bottle (mL) |
Material (Membrane) |
Pore Size (μm) |
Membrane Dia (mm) |
Membrane Color/Grid |
No. per Case |
| 121-0020* | 115/115 | CN | 0.2 | 50 | White/None | 72 |
| 121-0045* | 115/115 | CN | 0.45 | 50 | White/BlackGrid | 72 |
| 125-0020 | 150/150 | CN | 0.2 | 50 | White/None | 12 |
| 125-0045 | 150/150 | CN | 0.45 | 50 | White/BlackGrid | 12 |
| 125-0080 | 150/150 | CN | 0.8 | 50 | White/None | 12 |
| 126-0020 | 250/250 | CN | 0.2 | 50 | White/None | 12 |
| 126-0045 | 250/250 | CN | 0.45 | 50 | White/BlackGrid | 12 |
| 126-0080 | 250/250 | CN | 0.8 | 50 | White/None | 12 |
| 450-0020 | 500/500 | CN | 0.2 | 75 | White/None | 12 |
| 450-0045 | 500/500 | CN | 0.45 | 75 | White/BlackGrid | 12 |
| 450-0080 | 500/500 | CN | 0.8 | 75 | White/None | 12 |
| 127-0020 | 500/1000 | CN | 0.2 | 75 | White/None | 12 |
| 127-0045 | 500/1000 | CN | 0.45 | 75 | White/BlackGrid | 12 |
| 127-0080 | 500/1000 | CN | 0.8 | 75 | White/None | 12 |
* 115 mL sizes do not have Rapid-Flow support plate design
Thermo Scientific™ Nalgene™ Rapid-Flow Sterile Disposable Filter Units – Nylon Membrane
Nalgene™ Rapid-Flow™ Sterile Disposable Filter Units with Nylon Membrane are recommended for use with solutions where very low extractables are desired, or for filtration of solutions containing weak
solvents such as alcohols.
details
- Nalgene Rapid-Flow membrane support system provides fast flow and high throughput
- Upper chamber separates from storage bottle for easy access to filtrate
- Leakproof† screw cap eliminates pH shift in storage bottle to ensure longer life of stored media
- Upper chamber of filter units is clearly marked with pore size, membrane type, catalog number, lot number and expiration date for traceability
- Padless membrane support minimizes foaming of proteinaceous samples
- Non-pyrogenic and non-cytotoxic
- Gamma radiation-sterilized and individually bagged for a 5-year sterile shelf life
Nylon Membrane
- Low extractables and naturally hydrophilic
- Contains no wetting agents and is alcohol resistant
- Red collar for easy identification of nylon membrane
Includes: Polystyrene cover; graduated upper chamber and storage bottle; integral membrane filter; cellulosic-plugged side arm; quick-disconnect tubing adapter.
Nalgene Rapid-Flow Sterile Disposable Filter Units
| Cat. No. | Capacity, Upper/Storage Bottle (mL) |
Material (Membrane) |
Pore Size (μm) |
Membrane Dia (mm) |
No. per Case |
| 150-0020 | 150/150 | Nylon | 0.2 | 50 | 12 |
| 150-0045 | 150/150 | Nylon | 0.45 | 50 | 12 |
| 153-0020 | 250/250 | Nylon | 0.2 | 50 | 12 |
| 153-0045 | 250/250 | Nylon | 0.45 | 50 | 12 |
| 151-4020 | 500/500 | Nylon | 0.2 | 75 | 12 |
| 151-4045 | 500/500 | Nylon | 0.45 | 75 | 12 |
| 154-0020 | 500/1000 | Nylon | 0.2 | 75 | 12 |
| 154-0045 | 500/1000 | Nylon | 0.45 | 75 | 12 |
| 163-0020 | 500/500 | Nylon | 0.2 | 90 | 12 |
| 164-0020 | 1000/1000 | Nylon | 0.2 | 90 | 12 |
Thermo Scientific™ Nalgene™ Rapid-Flow Sterile Filter Storage Bottles
Nalgene™ Rapid-Flow™ Sterile Filter Storage Bottles are radiationsterilized to save you time and minimize contamination.
Use with Nalgene Bottle-Top Filters, or as replacements or extra receptacles for Nalgene Tissue Culture and Sterilization Filter Units. Excellent as handy sterile single-use storage containers.
details
- Headspace allows extra serum or other additives to be added to the filtrate
- Leakproof† screw caps to eliminate pH shift in bottle for longer life of stored media
- Sterilized by gamma radiation and individually bagged for a 5-year sterile shelf life
- Non-pyrogenic and non-cytotoxic
Nalgene Rapid-Flow Series Sterile Filter Storage Bottles
| Cat. No. | Storage Bottle Capacity (ml) |
No. per Case |
| 455-0150 | 150 | 24 |
| 455-0250 | 250 | 24 |
| 455-0500 | 500 | 12 |
| 455-1000 | 1000 | 12 |
